亚洲av无码无在线观看红杏,かしこまりました中文在线,国产精品免费一区二区三区视频,老同学3免费完整版在线观看

您好,歡迎進入上海力敏實業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> sigma現(xiàn)貨代理Dispase II D-4693

sigma現(xiàn)貨代理Dispase II D-4693

  • 更新時間:  2023-07-26
  • 產(chǎn)品型號:  
  • 簡單描述
  • sigma現(xiàn)貨代理Dispase II D-4693
    Related CategoriesApplication Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
    More...
    activity ≥0.5 units/mg solid
    sol
詳細介紹

sigma現(xiàn)貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[3] It has also been used for separating visceral yolk sac layers.[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現(xiàn)貨代理Dispase II D-4693

我公司代理sigma公司試劑,大量現(xiàn)貨,和選購


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數(shù)字),如:三加四=7
中文字幕在线无码一区二区三区| 色又黄又爽18禁免费视频| 精品国产av无码一区二区三区| 性色A∨亚洲一区二区三区| 偷玩朋友熟睡人妻| 丰满白嫩少妇肉肉大HD | 国产av无码专区亚洲av毛片搜 | 亚洲色无码a片一区二区潘甜甜| 久久久久成人精品| 欧美在线 | 亚洲| 蜜桃成人免费视频在线播放| 亚洲va国产va天堂va久久| 国产免费无码一区二区视频| gogogo日本免费观看电视| 精品人妻无码区在线视频| 无码OL丝袜高跟秘书在线观看| 精品国产日韩欧美一区二区三区| 亚洲阿v天堂在线2017| 亚洲精品毛A片久久久| 欧美又大又粗无码视频| 日本高清色www在线观看视频| 国产人妻人伦精品无码.麻豆| 国产一区二区三区在线播放| 好先生在线观看免费完整版| 性a欧美片| 狠狠躁夜夜躁无码中文字幕| 在线亚洲人成电影网站色www| 国产亚洲精品久久久久| 亚洲精品色情app在线下载观看| 久久精品国产亚洲一区二区| 九色porny丨国产首页注册| 国产精品久久久久久精品三级| 丁香花视频在线观看| 久久人妻av中文字幕| 国产精品久久久久久| 狼人无码精华av午夜精品| 中国人体艺术视频| 色综合色综合久久综合频道88 | 拔萝卜高清在线视频观看| 换着玩人妻hd中文字幕| 精品久久久久久无码人妻蜜桃|